BD FACSAria - Page 226

226 BD FACSAria User’s Guide
Center stream image is
dim or not visible in the
Side Stream window
Camera window is dirty Clean the lower camera window.
See Cleaning the Camera
Windows on page 211.
Stream is not intercepting
the diode laser
Adjust the micrometer dial on the
laser to make the stream intercept
as bright as possible. See
Figure 1-17 on page 41.
Center stream is off
center when the plate
voltage is turned on
Voltage center too low or too
high
Adjust the Voltage Center slider to
put the center stream back to
center.
Saline spray on deflection
plates or in sort block
Clean the deflection plates and the
area around them.
Arcing between
deflection plates
Plates are too close together
at the top
Adjust the plates so there is a gap
of approximately 0.28 in. (0.7 cm)
between them at the top.
Salt bridge Clean and dry the deflection plates
and the area around and behind
the plates.
ACDU sorting failure Insufficient stream voltage Increase the voltage for the far left
stream.
Splash shield not installed Install the splash shield. See
Installing the Sorting Hardware
on page 169.
Side stream position
reversal, where the
streams appear to be
associated with the
wrong voltage slider.
Voltage sliders are set too far
in or too far out.
Move sliders in or out so they
control the correct side streams.
Sorting Troubleshooting (continued)
Observation Possible Causes Recommended Solutions