Chapter 7: Troubleshooting 227
No deflection or
insufficient deflection
Insufficient voltage • Increase the plate voltage.
• Increase the side-stream voltages
using the slider controls.
Stream-charging wire is loose
or missing
Verify the stream-charging wire is
inserted all the way into the barb.
Salt bridge Turn off the stream. Remove the
nozzle. See Changing the Nozzle
on page 205 for instructions.
Plates are too far apart at top Adjust the plates so there is a gap
of approximately 0.28 in. (0.7 cm)
between them at the top.
Sorting paused because actual
Drop 1 value is out of range
Wait until the Sweet Spot adjusts
the amplitude to achieve the
Drop 1 target.
If this happens repeatedly during
sorting, there might be debris in
the nozzle or flow cell. See
Troubleshooting the Stream on
page 220 for suggestions.
Sorting Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
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