228 BD FACSAria User’s Guide
Fanning around center
or side streams
Nozzle inserted improperly Turn off the stream. Remove the
nozzle and ensure that the O-ring
is in place. Re-insert the nozzle at
a slight downward angle to
prevent loss or movement of the
O-ring. Make sure the nozzle is
completely seated against the back
wall of the cuvette flow cell.
Sweet Spot is off Turn on the Sweet Spot.
Incorrect sort precision mode Verify that the sort precision mode
is appropriate for your sorting
requirements. See Sort Precision
Modes on page 75.
2nd, 3rd, or 4th Drop values
not optimized
Adjust the 2nd, 3rd, and 4th Drop
settings to tighten the center
stream and fine-tune the side
streams.
Particles too big for nozzle Change the nozzle. See Changing
the Nozzle on page 205.
Sort button disabled Current tube pointer not set
to current tube
Click to move the current tube
pointer to the appropriate tube.
Population not listed in
Add menu on sort layout
Population defined using
snap-to gate
Redefine the population using
another gate type.
Viewing sort layout for
another tube
Open or create a sort layout for
the current acquisition tube.
Sort layout counters not
updating
Viewing sort layout for
another tube
Open or create a sort layout for
the current acquisition tube.
Sorting Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
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