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BD FACSAria - Page 75

BD FACSAria
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Chapter 2: Theory of Operation 75
Phase Masks cannot be used in conjunction with Yield Masks. Therefore, when
the Phase Mask is greater than zero, the Yield Mask automatically reverts to
zero.
Sort Precision Modes
Mask values can be combined in many different ways. By default, six Sort
Precision modes are already defined—Purity, 4-Way Purity, Yield, Single Cell,
Initial, and Fine Tune.
In Purity mode, the Yield Mask is set to the maximum to obtain the
greatest number of particles. Because the Purity Mask is also set to the
maximum, only drops free of contaminating particles will be sorted.
Sorting in Purity mode results in a sorted sample that is highly pure, at the
expense of recovery and yield.
In 4-Way Purity mode, the Purity Mask is set to the maximum, so only
drops free of contaminating particles will be sorted. The Yield Mask is set
to zero to ensure that residual charges from adjoining drops do not degrade
Precision Mode
Purity
4-Way
Purity Yield
Single
Cell Initial
Fine
Tune
Yield Mask320320320
Purity Mask 32 32 0 32 0 0
Phase Mask 0 0 0 16 0 0
Single Cell
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