76 BD FACSAria User’s Guide
the quality of side streams. The 4-Way Purity mode is recommended for
four-way sorting where precise deflection is required.
• In Yield mode, only the Yield Mask is used at its maximum value. Recovery
and yield are optimized at the expense of purity.
• In Single Cell mode, the Purity Mask is set to the maximum, so only drops
free of contaminating particles will be sorted. The Phase Mask is set to half
the maximum, so only particles centered within the sorted drop are
deflected. Drop trajectory and count accuracy are optimized at the expense
of yield. This mode is recommended for single-cell sorting or situations
where precise counting is required.
Tip Select the Single Cell checkbox to obtain the highest quality side
streams and the most accurate counts. When the checkbox is selected, drops
containing two target events (acceptable with a Purity Mask) are discarded.
The Yield Mask is disabled when Single Cell is selected.
The remaining modes are used mainly during drop delay determination. Initial
mode is equivalent to Yield mode, but it is named differently as a reminder to use
this as the initial mode when using Accudrop to set the drop delay.
• In Initial mode, only the Yield Mask is used at its maximum value.
Recovery and yield are optimized at the expense of purity.
• In Fine Tune mode, all masks are set to zero to deflect the maximum
number of drops. This mode is used to fine-tune the drop delay value. See
Determining the Drop Delay on page 158 for more information.
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