Chapter 7: Troubleshooting 237
Fluidics Troubleshooting
Observation Possible Causes Recommended Solutions
No fluid in line during
system prime
Air lock in bubble or fluidics
filter
Remove the filter for the
corresponding fluid, install bypass
tubing, and run Prime After Tank
Refill. Repeat the priming
procedure until you see fluid in
the line.
When fluid is running through the
line, remove the bypass tubing,
install the filter, and repeat the
priming procedure one last time.
Long clean fails Air lock in filter See previous recommendations.
Fluid line is detached Verify the fluid line connections
on the fluidics cart and on the
cytometer. Push firmly on each
line to ensure it is connected.
Fluidics cart air flow
<70 psi
Air leak Contact your BD Biosciences
service engineer.
Fluidics cart air flow
>100 psi
Regulator not adjusted
properly
Contact your BD Biosciences
service engineer.
Fluid leak under fluidics
cart or below side door
Condensation from pressure
relief valve
This is a normal phenomenon that
occurs when water is condensed
from room air. Condensation is
greater in humid environments. To
avoid slipping, check and wipe up
the water daily.
Bleeder valve is open Check and close all bleeder valves.
See Purging Filters on page 198.
Broken fluid line Contact BD Biosciences.
Fluid leaks at bubble
filter ports
Defective filter or loose
connection
Replace the bubble filter. See
Changing the Bubble Filter on
page 203.
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