46 BD FACSAria User’s Guide
Fluid Movement
The fluidics system is responsible for moving particles from the sample injection
chamber through the cuvette flow cell for interrogation, and then to waste or into
a collection device during sorting (Figure 2-1). The following sections describe
the controls used to move fluids through the BD FACSAria fluidics system.
Figure 2-1 Fluidic movement via the fluidics system
Sheath Flow
When you turn on the cytometer, the fluidic pumps start up. The fluidics system
is activated when you choose the Fluidics Startup command in BD FACSDiva
software. During fluidics startup, sheath fluid is pumped from the sheath
container through a 0.22-μm filter into the plenum reservoirs. The reservoirs are
pressurized to deliver sheath fluid to the cuvette flow cell at a constant pressure.
You can view the current sheath pressure using the Cytometer > Sheath Pressure
command (Figure 2-2 on page 47).
Sheath Flow
sheath tank
Sample Flow
plenum reservoirs HPLC valve
interrogation point
waste aspirator
sample tube
sample collection
chamber
cuvette flow
cell
sample injection
chamber
collection tube
or plate
sheath filter
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