134 BD FACSAria User’s Guide
be addressed. To track cytometer performance over time, copy means and CVs
for each parameter onto a QC log. See the Sample QC Log on page 150.
You can also perform Batch Analysis, which allows you to automatically export a
statistics file in comma separated value (CSV) format for a group of tubes. The
CSV file can be opened in a spreadsheet application. See Performing Batch
Analysis on page 149 for instructions on this process.
Data Collection
Before you record data for a sample, cytometer settings should be optimized to
position the cells of interest on scale for scatter and fluorescence parameters.
In general, the following settings are optimized for each sample type and
fluorochrome used:
• FSC and fluorescence area scaling
• FSC and SSC voltages
• FSC threshold
• Fluorescence PMT voltages
• Compensation
The following sections describe how to use previously optimized application
settings for a 4-color experiment. See Appendix B for more information. Also
refer to the Cytometer Setup and Tracking Application Guide for information on
running baselines and other details on using the CS&T module.
Compensation will be automatically calculated using the Compensation Setup
feature. For more information about this feature, refer to the BD FACSDiva
Software Reference Manual. If you are performing compensation manually, not
all steps will apply.
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