110 BD FACSAria User’s Guide
• Click the Sorting button ( ) on the Workspace toolbar to display the
Breakoff and Side Stream windows.
• Click the Stream button ( ) in the Breakoff window to turn on the
stream.
9 Open the sort block door and check the stream position in the waste
aspirator.
The stream should flow smoothly from the nozzle into the center of the
waste aspirator.
If the stream is flowing but is unsteady, check for bubbles in the flow cell. If
you see bubbles, turn off the stream, wait for the bubbles to float to the
top, and turn on the stream again.
If you see any dripping or spraying, or the stream image appears abnormal,
turn off the stream and see Troubleshooting the Stream on page 220.
10 Close the sort block door.
11 Select Sort > Sort Setup and verify the setup mode or select a new mode.
For information about Sort Setup modes, see Sort Setup on page 94.
Setting Up the Breakoff
Follow these steps to set the breakoff.
1 Adjust the Ampl. slider control until the drop breakoff is approximately in
the center of the Breakoff window.
Do not exceed 70 volts. If you cannot achieve a drop breakoff at <70 volts,
do the following:
• Check the flow cell for air bubbles. If you see bubbles, turn the stream
off and back on.
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