200 BD FACSAria User’s Guide
Changing the Sample Lines
The primary sample line between the sample injection chamber and the HPLC
valve should be changed every 4–6 months or when decreased event rates
indicate that the sample line might be clogged. The secondary sample line
between the HPLC valve and the cuvette flow cell needs changing only when it is
kinked or clogged.
To withstand the high pressures generated by the BD FACSAria flow cytometer,
the sample lines are attached at each end using a two-piece compression fitting,
where a cone-shaped ferrule is compressed onto the tubing as the connecting nut
is tightened.
To replace the tubing, you will need a length of replacement tubing from the
accessory kit. The 12-inch length of tubing is used for the primary sample line.
The 7-inch length is used for the secondary line (Figure 6-4). The ferrules and
connecting nuts can be reused when the tubing is replaced.
Figure 6-4 Sample lines and HPLC valve
All biological specimens and materials coming into contact with them can
transmit potentially fatal disease. Handle used tubing and fittings as if
capable of transmitting infection. Wear suitable protective clothing and
gloves.
HPLC valve
primary sample line
connecting nut
secondary
sample line
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