Chapter 1: BD FACSAria Cytometer Components 41
Stream-Viewing Optics
The BD FACSAria cytometer is equipped with additional optical components
that are used to view the stream (Figure 1-17).
• The upper camera generates an image used to monitor drop formation. It is
focused on the stream, just below the nozzle, to provide an image of the
drop breakoff.
• The lower camera generates an image used for the BD FACS™ Accudrop
*
option. It enhances the ability to see side streams and assists in setting an
accurate drop delay value.
Figure 1-17 Stream-viewing optics
Special image-processing software allows you to view the stream images from
each camera within separate windows in BD FACSDiva software. See Sorting on
page 62 for more information about viewing the streams and to learn how
Accudrop components are used to determine the drop delay.
* US Patent No. 6,372,506
upper
camera
diode laser
lower camera
viewing window
micrometer dial
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