Chapter 3: Using BD FACSDiva Software 95
copies of the corresponding original setups with the same defaults. This will
provide users with the flexibility to create two sets of sort values (pressure for
example) for the same nozzle size.
Do not use a nozzle size that is different from the sort setup setting. The gap
values and sensitivity algorithms are optimized for nozzles and are not
suitable for other nozzle sizes. The system performance could be unstable.
Table 3-1 Default Sort Setup values
BD FACSDiva v6.1 Setting 70 micron 85 micron
a
100 micron 130 micron
a
BD FACSDiva v6.0 Setting High Medium Low Custom
Sheath Pressure 70 45 20 10
Amplitude 60 32 12 24
Frequency 87 47 30 12
Drop 1 150 150 150 150
Gap (upper limit) 6 (14) 7 (17) 10 (21) 12 (21)
Attenuation Off Off Off Off
Drop Delay 47.00 30.00 27.00 16.00
Far left voltage 100 100 80 60
Left voltage 40 35 30 20
Right voltage 40 35 30 20
Far right voltage 100 100 80 60
Plate voltage 4,500 4,000 2,500 2,000
2nd Drop 20 20 10 0
3rd Drop 10 10 5 0
4th Drop 0 0 0 0
Laser Delay (blue) 0.00 0.00 0.00 0.00
To Next Page
Loading...
