Chapter 2: Theory of Operation 57
Neutral Density Filters
Neutral density (ND) filters transmit a fixed percentage of light, reducing the
transmitted intensity of all wavelengths equally. ND filters are neutral with
respect to wavelength.
The ND filters on the BD FACSAria allow approximately 10% of the light to be
transmitted. You can find them in front of the FSC and SSC detectors.
For applications involving small particles (eg, bacteria or platelets), you might
need to remove the FSC ND filter. See Removing/Installing the FSC ND Filter on
page 254. For applications involving large particles where events appear off scale
on the FSC axis with a gain of zero, the FSC ND filter is needed to decrease the
FSC signal and keep the events on scale.
ND1
10%
100%
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