56 BD FACSAria User’s Guide
Figure 2-10 Bandpass (BP) vs discriminating (DF) filters
In the detector arrays, DF filters block high-intensity laser light and filter the
remaining light to ensure that only the required wavelengths reach their intended
detector. For example, in the octagon array, PMT-A has a 780/60 DF filter in
front of it, which transmits light of 750–810 nm. Thus, the only wavelengths that
will reach the A detector are those between 750 and 810 nm.
For optimal detection of fluorescent light, a bandpass filter must always be
installed in front of each detector. For a list of the bandpass filters used in the
detector arrays, see Table 1-1 on page 40.
wavelength (nm)
% transmission
BP 500/50 filter
DF 500/50 filter
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