Chapter 3: Using BD FACSDiva Software 85
Figure 3-3 Levels indicators
When the sheath is low or the waste is full while the stream is running, the
corresponding indicator turns red and the following warning message is
displayed every 5 minutes until the stream is turned off. If the warning message is
not dismissed, the system automatically turns off the stream after 15 minutes.
Do not dismiss the warning message without refilling the sheath or
emptying the waste. If you continue to run the cytometer after dismissing
the message, the waste container could overflow or the sheath container
could run dry.
If the cytometer is run until the sheath supply is depleted, air will
accumulate in the bubble filter. Purge the filter as described in Purging the
Bubble Filter on page 214. If you are unable to remove all of the air, replace
the bubble filter as described in Changing the Bubble Filter on page 203.
ethanol
bleachDIwaste
sheath
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