210 BD FACSAria User’s Guide
Wipe it gently with optical lens paper. Make sure no fibers are left behind.
6 Use the wooden end of a swab to re-install the O-ring on the nozzle tip.
7 Carefully reinsert the nozzle into the flow cell.
Insert it at a slight downward angle to avoid contact between the O-ring
and the cuvette. Push it gently all the way forward without rocking it from
side to side. Ensure that the nozzle is in place and the O-ring is seated
properly.
8 Turn the nozzle-locking lever clockwise to the 12:00 position.
9 Turn on the stream and make sure it flows through the nozzle properly.
10 Close the flow cell access door.
Note that after re-installing the nozzle, you might need to change the angle of the
sort block to re-center the stream in the aspirator. To do so, loosen the
adjustment screws on either side of the deflection plates and rotate the sort block
(see Figure 1-11 on page 32). Tighten the screws when the stream is centered in
the aspirator. For further assistance, see Troubleshooting the Stream on page 220.
To Next Page
Loading...
