190 BD FACSAria User’s Guide
11 (Optional) When startup is complete, turn on the stream and let it run for
20–30 minutes.
Cleaning the Plenum Reservoirs
After you run a long clean with bleach or ethanol, you can clean the plenum
reservoirs by wiping them down with bleach or DI water. Perform the following
procedure to remove and clean the reservoirs.
1 Turn off the cytometer main power.
2 Open the side door and verify that the plenum reservoirs are empty.
3 Place absorbent paper under the plenum reservoirs and remove each one by
unscrewing it from its receiver.
NOTE The reservoirs must be reinstalled in the same locations. To keep
track, use an indelible marker to label them Left and Right when you
remove them.
4 Pull off the tubing connected to each reservoir.
5 Wipe the reservoirs with 10% bleach, followed by DI water.
After wiping, dry them with a clean, lint-free cloth.
To prevent damage to the plenum reservoirs, do not autoclave them.
reservoir
receiver