Chapter 6: Shutdown and Maintenance 191
6 Clean the float and receiver for each plenum reservoir.
Use a spray bottle to clean the floats. Use a cloth soaked with bleach or
ethanol to wipe down the receivers.
Dry each float and reservoir with a clean, lint-free cloth.
7 Reinstall the plenum reservoirs in their original locations.
Screw each reservoir into its receiver, making sure that the O-rings are
seated properly within each plenum reservoir. Reattach the tubing to the
bottom of each reservoir.
Clean Bulk Injection Chamber
Use the Clean Bulk Injection Chamber option to flush the injection chamber with
DI water and ethanol.
Allow 5–10 minutes to complete this cleaning procedure.
1 Ensure fluid levels are adequate. Empty the waste container if it is full.
This procedure requires approximately 250 mL each of DI water and
ethanol.
2 Open the flow cell access door and turn off the stream.
3 Remove the tube holder from the loading port.
It can be cleaned separately by wiping it down with ethanol, if needed.
4 Select Cytometer > Cleaning Modes > Long Clean.
If you have not already turned off the stream, a message appears reminding
you to do so.
The floats are fragile. Handle them with care. Do not spray ethanol into the
cytometer behind or below the plenum reservoirs.
To Next Page
Loading...
