BD FACSCelesta Flow Cytometer User’s Guide48
See Maintenance overview (page 46) for other recommended
cleaning solutions.
3. Move the tube support arm under the tube (vacuum off) and
allow the cleaning solution to run for 5 minutes with the
sample flow rate set to HIGH.
4. Repeat steps 2 and 3 with 1.5% BD Detergent Solution
Concentrate.
Note: The BD Detergent Solution Concentrate must be diluted
before use. 1.5% BD Detergent Solution is made from BD
Detergent Solution Concentrate, 15 mL diluted to make 1 L
total.
5. Repeat steps 2 and 3 with DI water.
6. Press the STANDBY button on the fluidics control panel.
7. Place a tube containing less than 1 mL of DI water on the SIP.
A tube with less than 1 mL of DI water should remain on the
SIP to prevent salt deposits from forming in the injection tube.
This tube also catches back drips from the flow cell.
Caution: Biohazard! Never mix BD Detergent Solution
and bleach because they can create dangerous fumes.
Caution! Do not leave more than 1 mL of water on
the SIP. When the instrument is turned off or left in
standby mode, a small amount of fluid will drip back
into the sample tube. If there is too much fluid in the
tube, it could overflow and affect the cytometer
performance.
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