Chapter 9: Troubleshooting 199
Sensitivity Test
Observation Possible Causes Recommended Solutions
FL1, FL2, FL3, FL4,
SSC, or FSC failure
Incorrect bead lot ID(s) Verify lot IDs in the Set Up
view.
HLA-B27 Calibration
Observation Possible Causes Recommended Solutions
>100-volt difference in
FL1 PMT voltage
Contaminated beads Prepare new beads.
Air bubble in flow cell Prime the fluidics (page 58).
Dirty flow cell or partial
clog
Perform monthly cleaning
(page 171).
Incorrect HLA-B27 bead
lot suffix
Enter correct bead lot ID and
suffix.
Calibration failure Incorrect HLA-B27 bead
lot suffix
Enter correct bead lot ID and
suffix.
Incorrect calibration beads Use only BD HLA-B27
calibration beads.
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