208 BD FACSCalibur Instructions for Use
Loader Troubleshooting
Observation Possible Causes Recommended Solutions
Sample tube not
pressurizing
Fluid mode not in RUN Press RUN.
Cracked sample tube Replace the sample tube.
No sheath pressure • Verify the vent valve is forward.
• Tighten the sheath cap.
• Check that all sheath connectors
are securely attached.
• Check for leaks or cracks in the
sheath reservoir. Replace, if
needed.
Outer sleeve missing or not
seated in retainer
1 Loosen the retainer.
2 Push up the outer sleeve until
fully seated.
3 Tighten the retainer.
Incorrect Tube Lifter
Parameters
Check lifter parameters in Loader
Manager software (page 180).
Compare values to those provided
by BD. If different, contact
BD Biosciences.
Difference between Loader
sealing and manual sealing
(Bal seal)
Reinstall Bal seal (page 186), and
manually check tube sealing. If
tube still does not seal, see
Instrument Troubleshooting on
page 190.
Worn Loader seal Replace the seal (page 182).
NOTICE Do this only after
checking all other troubleshooting
solutions.
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