BD FACSCalibur - Page 207

Chapter 9: Troubleshooting 207
Subset populations
outside attractors for all
samples
Compensation set incorrectly Rerun BD FACSComp
software, if necessary, and
optimize settings for
application (all except
compensation). Rerun
sample.
Incorrect attractor position Manually adjust attractor
and save as user-defined
reagent (page 134).
BD Multiset Results (continued)
Observation Possible Cause Recommended Solutions
CD3 PerCP
CD4 FITC
CD4 FITC
SSC-H
CD8 PE
SSC-H