BD FACSCalibur - Page 206

206 BD FACSCalibur Instructions for Use
Fluorescence parameters
dimmer than expected
Insufficient quantity of
reagent during sample
preparation
For BD Biosciences
reagents, re-stain using
20 μL reagent. Refer to the
information provided by
the manufacturer for
staining instructions.
For custom reagents,
titrate antibody to
determine sufficient
amount.
Donor-specific anomaly Wash sample before
staining.
BD Multiset Results (continued)
Observation Possible Cause Recommended Solutions
CD45 PerCP
SSC-H
CD3 FITC
CD8 PE
CD3 FITC
SSC-H
CD8 PE
CD4 APC