BD FACSCalibur - Page 205

Chapter 9: Troubleshooting 205
Low side scatter for
granulocytes in CD45 vs
SSC plot; no distinct
monocyte population
Aged blood and/or stained
cells
Refer to the information
provided by the reagent
manufacturer for stability
limitations.
BD Multiset Results (continued)
Observation Possible Cause Recommended Solutions
CD45 PerCP
SSC-H CD8 PE
CD3 FITC
CD3 FITC
SSC-H