92 BD FACSCalibur Instructions for Use
• Click the up or down arrow to change the Amp Gain value by .01.
• Press the Option key and click the arrow to change the value by 0.1.
• To change the value in larger increments, click between the arrows and
drag the slider control.
5 In the Threshold window, select FSC-H as the primary parameter.
6 Adjust the FSC Threshold Value to remove most of the debris without
cutting off the lymphocytes.
9
7 Click Pause, and then Abort in Acquisition Controls.
8 (Optional) Remove the isotype control tube from the SIP.
; Tip Remove the tube to conserve sample.
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