BD FACSCalibur - Page 192

192 BD FACSCalibur Instructions for Use
Low sample event rate Threshold too high Lower the threshold.
Insufficient gain for
threshold parameter
Increase the Amp Gain for the threshold
parameter (page 90).
Improperly mixed
sample
Mix sample to suspend cells.
Sample too dilute Concentrate the sample.
If the flow rate is not critical to the
application, set the flow rate to MED or
HI.
Clogged SIT Run 10% bleach and warm DI water for
20 minutes, then DI water for 10 minutes.
Erratic event rate Cracked sample tube Replace the sample tube.
Worn Bal seal Replace the Bal seal (page 176).
Partially blocked SIT • Remove the sample tube to allow
backflushing.
• If the clog persists, clean the SIT
(page 165).
Contaminated sample Prep the specimen again, making sure the
tube is clean.
Acquisition Troubleshooting (continued)
Observation Possible Causes Recommended Solutions