Chapter 9: Troubleshooting 193
Scatter parameters
distorted
Incorrect instrument
settings
Rerun BD FACSComp or optimize the
settings (Chapter 4).
Air bubble in flow cell Prime the fluidics (page 58).
Air in sheath filter Vent air from the sheath filter. See
page 57, step 14.
Dirty flow cell Perform monthly cleaning (page 171).
Air leak at sheath
reservoir
• Check the connectors on the sheath
sensor and saline filter.
• Check the Status sample voltage, in
RUN with test tube off (page 112). If
<10.2, replace the sheath tank, the cap,
and then the gasket.
Hypertonic buffers Check the pH of buffers and fixative.
Acquisition Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
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