194 BD FACSCalibur Instructions for Use
High CVs Air bubble in flow cell Prime the fluidics (page 58).
Sample flow rate on HI Set the sample flow rate to LO or MED.
Improper sheath
pressure
• Ensure the sheath cap is tight and all
connectors are secure.
• Check for leaks or cracks in the sheath
reservoir. Replace it, if needed.
• Check the Status sample voltage, in
RUN with test tube off (page 112).
Dirty flow cell Perform monthly cleaning (page 171).
Questionable sample
preparation
Verify sample preparation technique.
Air in sheath filter Vent air from the sheath filter. See
page 57, step 14.
Sample not diluted in
same fluid as sheath
Dilute the sample in the same fluid as
sheath. If you are running BD Calibrite
beads, dilute them in BD FACSFlow
solution and use BD FACSFlow for
sheath.
Acquisition Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
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