112 BD FACSCalibur Instructions for Use
Checking Cytometer Status
When you are connected to the cytometer, use the Status window to check
cytometer status during acquisition.
To access the window, choose Cytometer > Status.
• For Status = NOT READY, verify
- the blue laser is warmed up (it must be on
5 minutes before you run)
- the sheath is full and/or waste is empty (Check the Sheath Fluid and/or
Waste Tank status fields.)
• For Status = STANDBY, see if the fluid mode is STNDBY.
If in RUN, verify
- a sample tube is on the SIP
- the tube support arm is under the tube
- the tube is fully pressurized
• For Status = READY and no events displayed, see Acquisition
Troubleshooting on page 191.
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