196 BD FACSCalibur Instructions for Use
>100-volt difference in
FL4 PMT voltage
Air bubble in flow cell Prime the fluidics (page 58).
Expired APC beads Check the expiration date.
Prepare new beads, if needed.
Labeled beads other than
APC in unlabeled tube
Prepare new unlabeled + APC
beads. Rerun BD FACSComp.
Incorrect target values Contact BD Biosciences for the
latest target values.
Incorrect APC bead
lot ID
Enter the correct APC bead lot
ID.
APC beads not detected
(4-color setup not
performed)
No APC beads in first
tube
Add APC beads.
Insufficient number of
APC beads
Gently invert the APC bead vial
and add a drop to the tube.
Red laser disabled Ensure the FL4 option is
selected in BDPAC (page 27).
FL4 PMT voltage too high Decrease the FL4 PMT voltage.
Different PMT voltages
between runs
Some runs with the
Loader and some without
Maintain consistency: run with
or without the Loader each
time. Always run samples using
the same acquisition method as
BD FACSComp.
PMT Adjustment (continued)
Observation Possible Causes Recommended Solutions
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