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BD FACSCalibur - Page 135

BD FACSCalibur
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Chapter 5: Running Samples Manually 135
You can move, resize, and reorient attractors, but you cannot delete them
or add new ones.
To move an attractor, drag the center point.
To change the orientation and length, drag an endpoint.
To change the size, drag an edge.
6 Click Analyze.
This confirms changes and recalculates results. The QC Message area of the
Lab Report indicates a manual gate has been set.
7 (Optional) Save attractor changes.
Expert lymph gate changes cannot be saved.
Choose File > Save Reagent.
Enter a unique name, or keep the default name.
•Click OK.
Changes are saved as a user-defined reagent that can be reused the next
time a tube with the same reagent combination is analyzed.

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