172 BD FACSCalibur Instructions for Use
This bypasses the sheath filter, allowing sheath fluid to travel directly to the
flow cell, then to the waste reservoir.
5 Pull the vent valve toward you to repressurize.
6 Install a tube containing 3 mL of cleaning solution on the SIP.
7 Run the cleaning solution on HI for 20–30 minutes.
8 Replace the cleaning solution reservoir with a reservoir containing DI water.
9 Install a tube containing 3 mL of DI water on the SIP, and run the DI water
on HI for 20–30 minutes.
; Tip If you will not be using your instrument for a week or longer, keep
DI water in the fluidics system until you use the instrument again.
10 Replace the DI water reservoir with the original sheath reservoir, and
reconnect the sheath filter.
11 Ensure the tube on the SIP has 1 mL of DI water, and press STNDBY.
12 Turn off the instrument if you are finished running samples.
Do not run bleach or detergent through the sheath filter. This can break
down the filter paper within the filter, requiring filter replacement and
resulting in paper fragments that could clog the flow cell.
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