BD FACSCalibur User Manual

202 BD FACSCalibur Instructions for Use
Absolute count results
extremely high
Donor-specific pathology Re-stain sample. If problem
occurs for absolute counts
using hematology values, use
reagent with BD Trucount
tube.
Pipetting error Use fully charged, calibrated
electronic pipette (or
equivalent) that delivers a
precise amount of sample.
Make sure sample does not
drip from pipette tip during
sample preparation.
Incorrect beads/pellet
value(s) for absolute count
beads in Lot IDs
Enter correct beads/pellet
value(s); reanalyze.
Beads not found. Reagent
with BD Trucount tube
selected, but sample
prepared without
BD Trucount tube.
Re-stain sample. Use reagent
with BD Trucount tube.
Cell and/or BD Trucount
bead settling
• Vortex tubes before
acquiring. Resuspend cells
if tubes have been sitting
too long.
• Adjust mix settings.
• Re-stain sample, if
necessary.
Absolute count bead
attractor missing bead
population
Adjust bead attractor at
Manual Gate view.
General BD Multiset Software (continued)
Observation Possible Cause Recommended Solution

BD FACSCalibur Specifications

General

Brand	BD
Model	FACSCalibur
Category	Measuring Instruments
Language	English