72 BD FACSCalibur Instructions for Use
Time-Delay Calibration (4-Color Setup)
If APC beads were detected, BD FACSComp software performs time-delay
calibration after the PMTs are set. Leave the unlabeled and APC beads on the
cytometer while it is in progress.
If calibration is successful, the following message appears, and the software
proceeds to the Compensation view.
If calibration is not successful, you have 5 seconds to choose from the following:
• Init PMT: repeat PMT adjustment
• Continue on to Compensation: proceed with failed settings
• Retry: run time-delay calibration again (See page 197 for troubleshooting.)
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