50 BD FACSCanto II Instructions for Use
Performing Automated Setup
Whether you run samples in BD FACSCanto clinical software or BD FACSDiva
software, use BD FACSCanto clinical software to run automated setup and QC.
During setup, detector voltages are adjusted to place setup beads at defined target
values, sensitivity values are measured, and spectral overlap values are calculated
and applied to compensate data for fluorescence spillover. Use the Levey-Jennings
feature in BD FACSCanto clinical software to automatically track cytometer
setup values over time, and to monitor cytometer performance and see shifts or
trends in parameters as they occur.
Run setup once every 24 hours, using BD FACS 7-color setup beads. The
software tracks the time between setups and displays it in the Status window. A
setup age of more than 24 hours appears in red. Running a successful setup resets
the timer.
NOTICE If the software fails to respond during acquisition and you need to
restart the software, perform a fluidics startup prior to resuming acquisition. See
BD FACSCanto Software General Issues on page 199 for more information.
time since
last setup
To Next Page
Loading...
