190 BD FACSCanto II Instructions for Use
Cytometer Troubleshooting
Observation Possible Causes Recommended Solutions
Flow cell will not fill Air in bubble filter For BD FACSCanto clinical
software, run the Bubble Filter
Purge command at least once (you
might need to run it several times).
When finished, run De-gas Flow
Cell.
For BD FACSDiva software, run
the Bubble Filter Purge and De-gas
Flow Cell command at least once
(you might need to run it several
times).
Fluidics cart power off Turn on the power to the fluidics
cart by resetting the fluidics cart
circuit breaker switch (Figure 1-7
on page 24). Always use the
cytometer power button, located
on the left side of the cytometer, to
turn the system off and on.
No pressure in plenum Check air supply components (see
Figure 10-9 on page 181).
• Check air supply connections.
• Check air supply tubing for
kinks.
Sheath line disconnected
1 Check cubitainer-to-fluidics
cart, and fluidics cart–to-
cytometer connections. See
Reconnecting the Fluidics Cart
Tubing on page 181.
2 Check tubing for kinks.
BD FACSFlow cubitainer
empty
Replace the BD FACSFlow
cubitainer. See Changing a
Cubitainer on page 166.
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