Chapter 11: Troubleshooting 191
Flow cell will not fill
(continued)
Air in BD FACSFlow filter
(fluidics cart)
Purge air from the filter. See
Priming Fluidics Lines on
page 169.
Air lock in BD FACSFlow
filter (on fluidics cart)
See Removing an Air Lock on
page 169.
No air pressure in cart • If the cart is attached to an
auxiliary air supply, switch on
auxiliary air.
• If the cart is not attached to an
auxiliary air supply, switch off
auxiliary air.
Fluid backfill into
sample tube
Cracked tube • Transfer sample to new tube.
• Make sure you are using
appropriate tubes. See System
Requirements on page 28.
Bal seal improperly installed
or worn
Reinstall or replace the Bal seal.
See Replacing the Bal Seal on
page 175.
Air lock in BD FACSFlow
filter
See Removing an Air Lock on
page 169.
Tube partially removed from
SIT after acquisition
Once you start to remove a tube
from the SIT, complete the action.
Low laser power
indication
Flow cell access door open Close the door completely.
Laser power output is below
requirement
Call BD Biosciences.
Backflushing of sheath
fluid into sample tube
Aspirator arm bumped or
moved to side, but tube was
not removed
1 Repeat tube removal sequence.
2 Perform a manual SIT flush.
Cytometer Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
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