BD FACSCanto II - No events in plots after clicking Acquire, NO acquisition indicator lights are blinking (no fluorescent signal); Fluorescent signal missing

Chapter 11: Troubleshooting 221
No events in plots after
clicking Acquire,
NO acquisition indicator
lights are blinking
Cracked tube • Transfer sample to new tube.
• Make sure you are using
appropriate tubes.
SIT clogged Clean the flow cell.
Current cytometer
configuration different
from optical setup
Verify the cytometer
configuration corresponds to the
cytometer’s optical setup. If it
does not, contact your System
Administrator.
Incorrect sample
preparation
Re-stain and re-run samples.
Indicator light switch off Turn indicator light switch on.
Laser delay set
incorrectly
Contact your lab manager to
correct the laser delay settings.
Area scaling factor set
incorrectly
Contact your lab manager to
correct area scaling factor
settings.
Fluorescent signal
missing
Current cytometer
configuration different
from optical setup
Verify the cytometer
configuration corresponds to the
cytometer’s optical setup, and
parameter list includes
appropriate parameters.
Laser delay set
incorrectly
Contact your lab manager to
correct the laser delay settings.
Area scaling set
incorrectly
Contact your lab manager to
correct the laser delay settings.
Fluorescence threshold
set too high
Adjust threshold.
Incorrect sample
preparation
Re-stain and re-run samples.
BD FACSDiva Software Acquisition (continued)
Observation Possible Causes Recommended Solutions