222 BD FACSCanto II Instructions for Use
Unexpected results after
clicking Next
Current tube pointer on
wrong tube
Put the current tube pointer in
front of the appropriate tube
before clicking Next.
Unexpectedly high event
rate
Threshold too low Increase the threshold.
Sample flow rate too
high
Decrease the flow rate.
Bubbles in flow cell Check for bubbles. If found, run
De-gas Flowcell.
Unexpectedly low event
rate
Threshold too high Decrease the threshold.
Erratic event rate Sample aggregates Filter the sample.
Distorted scatter
parameters
Cytometer settings
adjusted incorrectly
Optimize the scatter parameters.
Bubbles in flow cell Check for bubbles. If found, run
De-gas Flowcell.
Excessive amount of
debris in plots
Threshold set too low Increase the threshold.
Dirty sheath filter Replace sheath filter.
Dirty flow cell Clean flow cell. See page 173.
High CVs Sample flow rate too
high
Decrease the flow rate.
Window extension set
incorrectly
Contact lab manager to set the
window extension to 7.0.
BD FACSDiva Software Acquisition (continued)
Observation Possible Causes Recommended Solutions
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