Chapter 11: Troubleshooting 223
Acquisition stops High event rate (>10,000
events/sec) with more
than 8 compensated
parameters
• Increase threshold.
• Decrease threshold channel
gain.
• Dilute sample.
• Define only channels of
interest in parameter list.
Fewer events than
expected in gated
population
Window extension set
incorrectly
Contact lab manager to set the
window extension to 7.0.
On-axis events left out of
gate
Include events on the axis.
Gates improperly set Check gating strategy.
Dirty sheath filter Replace sheath filter.
Dirty flow cell Clean flow cell. See page 173.
High electronic abort
rate (>10% of system
event rate)
Event rate too high Decrease the flow rate.
Sample too concentrated Dilute the sample.
Threshold set too low Increase the threshold.
Window extension set
incorrectly
Contact lab manager to set the
window extension to 7.0.
Increasing threshold
results in decreased area
signal
Window extension set
too low
Set the window extension to 7.0
Area measurement off-
scale while height
measurement is on scale
Area scaling set too high Contact your lab manager to
correct area scaling settings.
BD FACSDiva Software Acquisition (continued)
Observation Possible Causes Recommended Solutions
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