84 BD FACSCanto II Instructions for Use
If the tube is not fully seated, the following error message appears. Click
OK. Remove the tube and repeat step 1 to properly reinstall it.
3 Adjust the FSC and SSC voltages to appropriately display the scatter
properties of the unstained control.
Figure 5-10 Voltages adjusted
4 Click the Threshold tab and adjust the FSC Threshold, if needed.
Set the threshold to remove most of the debris without cutting off the
lymphocyte population (Figure 5-10).
5 Adjust the P1 gate on the Unstained Control worksheet to surround only
the lymphocyte population (Figure 5-10).
Select the gate by clicking on the boundary. Once selected, you can drag the
gate to move it, or drag any of the selection handles to change the gate
shape and size.
6 Once the gate is adjusted, right-click its boundary and choose Apply to All
Compensation Controls.
This applies your gate changes to the P1 gates on the remaining
compensation worksheets.
To Next Page
Loading...
