202 BD FACSCanto II Instructions for Use
Tube pressurization
errors
Cracked tube • Transfer sample to new tube.
• Make sure you are using
appropriate tubes.
Drop at top of tube Dry the inside of the tube with a
cotton swab and re-run.
Bal seal improperly
installed or worn
Reinstall or replace the Bal seal.
See page 175 for instructions.
Loader misaligned
1 Try running in manual mode
instead of automatic. See
step 2 on page 51 for
converting to manual mode.
2 If unsuccessful, call
BD Biosciences.
Wrong tubes used Make sure you are using
recommended tubes. See System
Requirements on page 28.
Tubing to fluidics cart
kinked or disconnected
Reconnect or straighten the
tubing. See page 181.
Tube not detected Tube not seated on Bal
seal properly
• Ensure that the tube is all the
way up onto the Bal seal.
• Ensure you are using
appropriate tubes. See System
Requirements on page 28.
• Replace or reinstall Bal seal.
Vacuum error Tubing to fluidics cart
kinked or disconnected
Reconnect or straighten the
tubing. See page 181.
BD FACSCanto Software General Issues (continued)
Observation or Error
Message
Possible Causes Recommended Solutions
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