BD FACSCanto II - No events in plots after clicking Run, NO acquisition indicator lights are blinking (no fluorescent signal); FCS file not created

208 BD FACSCanto II Instructions for Use
No events in plots after
clicking Run,
NO acquisition indicator
lights are blinking
(no fluorescent signal)
Cracked tube • Transfer sample to new tube.
• Make sure you are using
appropriate tubes.
SIT clogged Clean the flow cell.
Current cytometer
configuration different
from optical setup
Verify the cytometer
configuration corresponds to the
cytometer’s optical setup.
Laser delay set
incorrectly
Contact BD Service.
Unexpectedly high event
rate
Threshold too low Increase the threshold.
Sample too concentrated Dilute the sample.
Bubbles in flow cell Check for bubbles. If found, run
De-gas Flowcell.
Unexpectedly low event
rate
Threshold too high Decrease the threshold.
SIT clogged Clean the flow cell.
Excessive amount of
debris in plots
Threshold too low Increase the threshold.
Dead cells or debris in
sample
Examine the sample under a
microscope.
Stained sample too old Check the reagent package
insert.
FCS file not created PC hard disk full
1 Delete unnecessary files.
2 Run disk utilities regularly.
BD FACSCanto Software Acquisition (continued)
Observation Possible Causes Recommended Solutions