BD FACSCanto II - Distorted populations or unexpected pattern in plot

Chapter 11: Troubleshooting 209
Acquisition stops High event rate (>10,000
events/sec) with more
than 8 compensated
parameters
• Increase threshold.
• Decrease threshold channel
gain.
• Dilute sample.
• Define only channels of
interest in parameter list.
Distorted populations or
unexpected pattern in
plot
Cytometer settings
adjusted incorrectly
Optimize the scatter parameters.
Bubbles in flow cell Check for bubbles. If found, run
De-gas Flowcell.
Flow cell dirty Clean the flow cell.
SIT not cleaned between
tubes
• Choose Cytometer >
Cleaning Modes > SIT Flush.
Incorrect gating Verify the gating.
BD FACSCanto Software Acquisition (continued)
Observation Possible Causes Recommended Solutions