Chapter 11: Troubleshooting 211
Insufficient beads
acquired (<500)
Cell concentration too
high
Dilute the sample, re-stain, and
run it again.
% T-sum failure Large number of double-
positive or double-
negative T cells
Inspect the gates and include all
required events. Adjust gates
manually, if needed.
Lymphosum failure Events incorrectly
classified as T, B, or NK
Inspect the gates and make sure
required events are included.
Adjust gates manually, if needed.
Laser power low during
recording: Visually
inspect.
Data collected with low
laser power
Inspect the data.
If the power is outside the
acceptable range, call
BD Biosciences.
Lymph gate failure: Gate
manually.
No usable lymph gate • Adjust the lymph gate
manually.
• If necessary, run the sample
again.
BD FACSCanto Software TBNK Analysis
QC Messages (continued)
Message
Possible Causes
Recommended Solutions
To Next Page
Loading...
