Chapter 11: Troubleshooting 213
BD FACSCanto Software Four- and Six-Color TBNK
(continued)
Observation Possible Causes Recommended Solutions
Debris encroaching on
populations in CD45 vs
SSC plot
• Excessive mixing
• Aged blood or stained
cells
Prepare sample again.
Vertically compressed
populations
Side scatter too low Reacquire sample. Set SSC so
granulocytes reach to top of
CD45 vs SSC plot.
Lipemic sample Check reagent package insert for
instructions.
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