214 BD FACSCanto II Instructions for Use
Indistinct populations;
events sparse or missing
from one population;
lack of separation
between CD3– and
CD3+ cluster
Incorrect spectral overlap Re-run setup, optimizing for
application. Re-run sample.
Granulocytes cut off at
top of plot; stretched
monocyte population
High SSC Re-run setup, optimizing for
application. Re-run sample,
lowering the SSC.
BD FACSCanto Software Four- and Six-Color TBNK
(continued)
Observation Possible Causes Recommended Solutions
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