Chapter 1: Introduction 17
Optics Components and Theory of Operation
Once the sample moves into the flow cell, particles move in single file through the
laser beams. The scattered and emitted light from these particles provides
information about their size, shape, granularity, and fluorescence properties.
From the flow cell, laser-excited and scattered light is routed to the detector
arrays, which consist of photomultiplier tubes (PMTs) arranged in one octagon
and one trigon (a 4-2 optical configuration).
FSC diode
where lasers
intercept
sample stream
obscuration
bar
flow cell
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