Chapter 11: Troubleshooting 193
Tube does not load, or
sample tube does not fit
snugly on SIT
Improper tubes used Use only uncapped 12 x 75-mm
BD Falcon polystyrene test tubes,
BD Trucount tubes, and BD FACS
7-color setup beads tubes. See
System Requirements on page 28.
Defective or cracked tube • Transfer sample to new tube.
• Make sure you are using
appropriate tubes. See System
Requirements on page 28.
Bal seal improperly installed
or worn
Reinstall or replace the Bal seal.
See Replacing the Bal Seal on
page 175.
Cytometer and fluidics
cart will not turn on
Power cord disconnected
from wall socket or cytometer
Reconnect the power cord to the
wall socket or the cytometer.
Cytometer circuit breaker
tripped
Switch on the cytometer circuit
breaker, followed by the
cytometer power (if off).
Blown fuse in cytometer
controller
Turn off the cytometer main
power and call BD Biosciences.
Fluid leaking from SIT or
aspirator arm
Interior valve failure or leak
1 Turn off the cytometer power.
2 Clean up the liquid, using
proper precautions.
3 Call BD Biosciences.
Waste line to fluidics cart
disconnected
1 Turn off the cytometer power.
2 Clean up the liquid, using
proper precautions.
3 Check that waste lines are
securely plugged in.
4 Turn on the cytometer power.
Cytometer Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
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