Chapter 4: Maintenance 111
Monthly Maintenance
When running the HTS daily, perform the following cleaning procedures at the
end of every month. If you are using the HTS less frequently, adjust the monthly
maintenance schedule accordingly.
• Monthly Cleaning—see the following section
• Surface Inspection and Cleaning—see page 114
Monthly Cleaning
BD LSR II Monthly Cleaning
To perform a monthly cleaning procedure, choose HTS > Monthly Clean. The
software prompts you to install a tank containing BD FACSClean (or a 10%
bleach solution) in place of the cytometer sheath tank, and then pumps cleaning
solution through the lines for 30 minutes. When cleaning is complete, the software
prompts you to install a rinse tank, and then pumps DI water through the lines for
30 minutes. Allow 60 minutes to complete the monthly cleaning procedure.
To perform the monthly cleaning you will need DI water and BD FACSClean or a
fresh 10% bleach solution (1 part bleach in 9 parts DI water).
1 Bypass the cytometer sheath filter:
• Press the quick-disconnectors on both sides of the filter assembly.
• Remove the filter assembly.
• Reconnect the two fluidic lines.
2 Remove the sheath tank and replace the sheath fluid with BD FACSClean
or a freshly prepared 10% bleach solution. Replace the tank.
To ensure that the 10% bleach solution retains its full germicidal effect,
prepare a fresh solution daily.
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